Редактирование: ALKBH8 (раздел)

==Publications==

{{medline-entry
|title=Loss of epitranscriptomic control of selenocysteine utilization engages senescence and mitochondrial reprogramming .
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/31765888
|abstract=Critically important to the maintenance of the glutathione (GSH) redox cycle are the activities of many selenocysteine-containing GSH metabolizing enzymes whose translation is controlled by the epitranscriptomic writer alkylation repair homolog 8 ([[ALKBH8]]). [[ALKBH8]] is a tRNA methyltransferase that methylates the wobble uridine of specific tRNAs to regulate the synthesis of selenoproteins. Here we demonstrate that a deficiency in the writer [[ALKBH8]] (Alkbh8 ), alters selenoprotein levels and engages senescence, regulates stress response genes and promotes mitochondrial reprogramming. Alkbh8  mouse embryonic fibroblasts (MEFs) increase many hallmarks of senescence, including senescence associated β-galactosidase, heterocromatic foci, the cyclin dependent kinase inhibitor p16 , markers of mitochondrial dynamics as well as the senescence associated secretory phenotype (SASP). Alkbh8  cells also acquire a stress resistance phenotype that is accompanied by an increase in a number redox-modifying transcripts. In addition, Alkbh8  MEFs undergo a metabolic shift that is highlighted by a striking increase in the level of uncoupling protein 2 (UCP2) which enhances oxygen consumption and promotes a reliance on glycolytic metabolism. Finally, we have shown that the Alkbh8 deficiency can be exploited and corresponding MEFs are killed by glycolytic inhibition. Our work demonstrates that defects in an epitransciptomic writer promote senescence and mitochondrial reprogramming and unveils a novel adaptive mechanism for coping with defects in selenocysteine utilization.
|mesh-terms=* AlkB Homolog 8, tRNA Methyltransferase
* Animals
* Cells, Cultured
* Cellular Senescence
* Epigenesis, Genetic
* Gene Deletion
* Gene Expression Profiling
* Humans
* Mice
* Mitochondria
* Oxygen Consumption
* Selenocysteine
* Uncoupling Protein 2
|keywords=* Epitranscriptome
* Mitochondria
* Selenium
* Senescence
* Uncoupling protein
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904832
}}
{{medline-entry
|title=ALKB-8, a 2-Oxoglutarate-Dependent Dioxygenase and S-Adenosine Methionine-Dependent Methyltransferase Modulates Metabolic Events Linked to Lysosome-Related Organelles and Aging in C. elegans.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/30338756
|abstract=ALKB-8 is a 2-oxoglutarate-dependent dioxygenase homologous to bacterial AlkB, which oxidatively demethylates DNA substrates. The mammalian AlkB family contains AlkB homologues denominated [[ALKBH1]] to 8 and [[FTO]]. The C. elegans genome includes five AlkB-related genes, homologues of [[ALKBH1]], 4, 6, 7, and 8, but lacks homologues of [[ALKBH2]], 3, and 5 and [[FTO]]. [[ALKBH8]] orthologues differ from other AlkB family members by possessing an additional methyltransferase module and an RNA binding N-terminal module. The [[ALKBH8]] methyltransferase domain generates the wobble nucleoside 5-methoxycarbonylmethyluridine from its precursor 5-carboxymethyluridine and its (R)- and (S)-5-methoxycarbonylhydroxymethyluridine hydroxylated forms in tRNA Arg/UCG and tRNA Gly/UCC. The [[ALKBH8]]/ALKB-8 methyltransferase domain is highly similar to yeast TRM9, which selectively modulates translation of mRNAs enriched with [[AGA]] and [[GAA]] codons under both normal and stress conditions. In this report, we studied the role of alkb-8 in C. elegans. We show that downregulation of alkb-8 increases detection of lysosome-related organelles visualized by Nile red in vivo. Reversely, forced expression of alkb-8 strongly decreases the detection of this compartment. In addition, overexpression of alkb-8 applied in a pulse during the L1 larval stage increases the C. elegans lifespan.
|mesh-terms=* Aging
* AlkB Enzymes
* Animals
* Animals, Genetically Modified
* Caenorhabditis elegans
* Caenorhabditis elegans Proteins
* Dioxygenases
* Down-Regulation
* Embryo, Nonmammalian
* Gene Expression Regulation, Developmental
* Green Fluorescent Proteins
* Ketoglutaric Acids
* Larva
* Longevity
* Lysosomes
* Methyltransferases
* Operon
* Promoter Regions, Genetic
* RNA Interference
* S-Adenosylmethionine


}}