Редактирование: VPS4A

Vacuolar protein sorting-associated protein 4A (EC 3.6.4.6) (Protein SKD2) (VPS4-1) (hVPS4) [VPS4]

==Publications==

{{medline-entry
|title=The expression changes of vacuolar protein sorting 4B ([[VPS4B]]) following middle cerebral artery occlusion (MCAO) in adult rats brain hippocampus.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/24077878
|abstract=Vacuolar protein sorting 4 (VPS4), is a member of ATPases associated with diverse cellular activities protein family. VPS4 is composed of [[VPS4A]] and [[VPS4B]], [[VPS4B]] plays an important role in the lysosomal degradation pathway, intracellular protein trafficking, virus budding and abscission of cytokinesis. However, information regarding its distribution and possible function in the central nervous system is limited. Therefore, we performed a middle cerebral artery occlusion (MCAO) in adult rats and detected the dynamic changes of [[VPS4B]] in hippocampus [[CA1]] subregion. We found that the [[VPS4B]] expression was increased strongly after MCAO and reached the peak after 3 days. [[VPS4B]] mainly located in the cytoplasm of neurons, but not astrocytes and microglia. Moreover, there was a concomitant up-regulation of active caspase-3. In vitro studies indicated that the up-regulation of [[VPS4B]] may be involved in oxygen-glucose deprivation-induced PC12 cell death. And knock-down of [[VPS4B]] in cultured differentiated PC12 cells by siRNA showed that [[VPS4B]] promoted the expression of active caspase-3. Collectively, all these results and MTT assay suggested that the up-regulation of [[VPS4B]] played an important role in the pathophysiology after MCAO, and further research is needed to have a good understanding of its function and mechanism.
|mesh-terms=* Aging
* Animals
* Apoptosis
* Biomarkers
* Blotting, Western
* CA1 Region, Hippocampal
* Caspase 3
* Cell Survival
* Gene Knockdown Techniques
* Glucose
* Hippocampus
* Hypoxia
* Immunohistochemistry
* Infarction, Middle Cerebral Artery
* Male
* Neurons
* PC12 Cells
* Rats
* Rats, Sprague-Dawley
* Up-Regulation
* Vesicular Transport Proteins

|full-text-url=https://sci-hub.do/10.1007/s10571-013-9989-5
}}