Редактирование: POU1F1

Pituitary-specific positive transcription factor 1 (PIT-1) (Growth hormone factor 1) (GHF-1) [GHF1] [PIT1]

==Publications==

{{medline-entry
|title=Neonatal Meishan pigs show [[POU1F1]] genotype effects on plasma GH and [[PRL]] concentration.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/11812632
|abstract=Chinese Meishan pigs develop rapidly with onset of puberty at less than 100 days of age, and have a smaller placental size and larger litter size as compared with British/Continental breeds. [[POU1F1]] is a member of the POU-domain family gene and is a positive regulator for growth hormone (GH), prolactin ([[PRL]]), and thyroid-stimulating hormone beta (TSHbeta) in several mammalian species. To investigate the role of [[POU1F1]] in controlling pig growth and reproduction traits, Meishan (MS) pigs segregating a MspI [[POU1F1]] polymorphism were used to determine differences of GH and [[PRL]] at both mRNA and circulating hormone concentrations. Animals from nine litters were used to collect pituitary (n=60) and/or blood samples (n=80) at day 0, 15, and 30 after birth, and all animals were genotyped (CC, CD, DD) for the MspI [[POU1F1]] polymorphism. Reverse transcriptase-polymerase chain reaction (RT-PCR) with standard curve quantification was used to quantify mRNA levels for GH, [[PRL]], and two alternative [[POU1F1]] transcripts, [[POU1F1]]-alpha, and [[POU1F1]]-beta. Radioimmunoassays were done to determine the circulating concentration of GH and [[PRL]] in blood plasma. Our results indicated a significant effect of [[POU1F1]] genotype on circulating levels of both GH and [[PRL]] at birth, but not thereafter. The DD neonates had lower levels of GH, but higher levels of [[PRL]], than other genotypes. [[POU1F1]]-alpha mRNA decreased (P<0.05) from days 0 to 30, which paralleled decreases (P<0.05) in GH mRNA as well as [[PRL]] and GH plasma levels over the same period. [[POU1F1]]-beta mRNA levels did not significantly change over this period. Correlations were significant between [[POU1F1]]-alpha mRNA and both GH mRNA and GH plasma concentration levels, as well as between the two [[POU1F1]] mRNA isoforms. Results from this study add to our understanding of the role of [[POU1F1]] in controlling pig development and reproduction.
|mesh-terms=* Aging
* Animals
* Animals, Newborn
* Female
* Genotype
* Growth Hormone
* Litter Size
* Male
* Polymorphism, Genetic
* Prolactin
* RNA, Messenger
* Radioimmunoassay
* Reverse Transcriptase Polymerase Chain Reaction
* Swine
* Thyrotropin
* Time Factors
* Transcription Factors

|full-text-url=https://sci-hub.do/10.1016/s0378-4320(01)00177-4
}}