Редактирование: NKX2-5

Homeobox protein Nkx-2.5 (Cardiac-specific homeobox) (Homeobox protein CSX) (Homeobox protein NK-2 homolog E) [CSX] [NKX2.5] [NKX2E]

==Publications==

{{medline-entry
|title=Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/30663680
|abstract=Myocardial infarction (MI) is a leading cause of morbidity and mortality around the world. A major goal of regenerative medicine is to replenish the dead myocardium after MI. Although several strategies have been used to regenerate myocardium, stem cell therapy remains a major approach to replenish the dead myocardium of an MI heart. Accumulating evidence suggests the presence of resident cardiac stem cells (CSCs) in the adult heart and their endocrine and/or paracrine effects on cardiac regeneration. However, CSC isolation and their characterization and differentiation toward myocardial cells, especially cardiomyocytes, remains a technical challenge. In the present study, we provided a simple method for the isolation, characterization, and differentiation of CSCs from the adult mouse heart. Here, we describe a density gradient method for the isolation of CSCs, where the heart is digested by a 0.2% collagenase II solution. To characterize the isolated CSCs, we evaluated the expression of CSCs/cardiac markers Sca-1, [[NKX2-5]], and [[GATA4]], and pluripotency/stemness markers OCT4, [[SOX2]], and Nanog. We also determined the proliferation potential of isolated CSCs by culturing them in a Petri dish and assessing the expression of the proliferation marker Ki-67. For evaluating the differentiation potential of CSCs, we selected seven- to ten-days cultured CSCs. We transferred them to a new plate with a cardiomyocyte differentiation medium. They are incubated in a cell culture incubator for 12 days, while the differentiation medium is changed every three days. The differentiated CSCs express cardiomyocyte-specific markers: actinin and troponin I. Thus, CSCs isolated with this protocol have stemness and cardiac markers, and they have a potential for proliferation and differentiation toward cardiomyocyte lineage.
|mesh-terms=* Aging
* Animals
* Biomarkers
* Cell Culture Techniques
* Cell Differentiation
* Male
* Mice
* Mice, Inbred C57BL
* Multipotent Stem Cells
* Myocardium
* Myocytes, Cardiac

|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7207148
}}