Редактирование: NDRG4

Protein NDRG4 (Brain development-related molecule 1) (N-myc downstream-regulated gene 4 protein) (Vascular smooth muscle cell-associated protein 8) (SMAP-8) [BDM1] [KIAA1180]

==Publications==

{{medline-entry
|title=Molecular characterization of [[NDRG4]]/Bdm1 protein isoforms that are differentially regulated during rat brain development.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/11978392
|abstract=We previously reported the identification of a novel gene, Bdm1/[[NDRG4]], that was expressed predominantly in the postnatal rat brain and might possibly play a role in this process. We describe here the characterization of a [[NDRG4]] protein in a developing and maturing rat brain. Antibody raised against glutathione S-transferase (GST)-[[NDRG4]] fusion protein recognized four protein species of 38, 39, 41, and 45 kDa on Western blotting of proteins from differently staged rat brains. The 38-kDa form was revealed after birth, and the amount of this species peaked on postnatal day 15. The 39-kDa form became detectable after postnatal week 6. The 41-kDa form appeared late in embryogenesis, increased by postnatal day 15, and disappeared at postnatal week 6. The 45-kDa form was abundant during the late embryonic period and slightly decreased after birth. Subcellular fractionation of cerebra indicated that the [[NDRG4]] protein was distributed mainly in the mitochondria and endoplasmic reticulum (ER). Detergent solubility assays and protease susceptibility demonstrated that in the ER [[NDRG4]] protein is membrane-associated and luminally oriented. The 45-kDa isoform was induced during [[NGF]]-mediated neuronal differentiation of PC12 cells, but not by tunicamycin which causes ER stress. Differential expressions of [[NDRG4]] protein isoforms may be a mechanism for modifying the [[NDRG4]] function and for the formation of a functioning nervous system.
|mesh-terms=* Aging
* Amino Acid Sequence
* Animals
* Brain
* Cell Differentiation
* DNA, Complementary
* Endoplasmic Reticulum
* Gene Expression Regulation, Developmental
* Molecular Sequence Data
* Muscle Proteins
* Nerve Tissue Proteins
* Neurons
* PC12 Cells
* Protein Isoforms
* Rats
* Reverse Transcriptase Polymerase Chain Reaction
* Subcellular Fractions
* Tissue Distribution

|full-text-url=https://sci-hub.do/10.1016/s0165-3806(02)00303-6
}}