Редактирование: DDT

D-dopachrome decarboxylase (EC 4.1.1.84) (D-dopachrome tautomerase) (Phenylpyruvate tautomerase II)

==Publications==

{{medline-entry
|title=Prognostic Value of a Test of Central Auditory Function in Conversion from Mild Cognitive Impairment to Dementia.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/32388503
|abstract=It has been suggested that central auditory processing dysfunction might precede the development of cognitive decline and Alzheimer's disease (AD). The Dichotic Digits Test ([[DDT]]) has been proposed as a test of central auditory function. Our objective was to evaluate the predictive capacity of the [[DDT]] in conversion from mild cognitive impairment (MCI) to dementia. A total of 57 participants (26 females) with MCI were tested at baseline with pure tone audiometry, speech in quiet and in noise, and the [[DDT]]. The cognitive outcome was retrieved from medical files after 5 years. Groupwise comparisons of the baseline [[DDT]] scores were performed and the relative risk was calculated. Altogether 22 subjects developed any kind of dementia. Of the original 57 individuals within the MCI group, 15 developed AD and 7 developed other types of dementia. There was no significant difference in baseline [[DDT]] scores between the participants who converted to AD and those who did not. However, the group who developed other types of dementia (especially frontotemporal dementia) had lower [[DDT]] scores in the left ear than those participants who did not develop dementia. With a baseline [[DDT]] score below 50% correct responses, the participants diagnosed with MCI had a 2.49-times-higher risk of developing dementia than those with scores of 50% or better. The [[DDT]] as a central auditory test may be suitable when evaluating cognitive decline.

|keywords=* Aging
* Alzheimer’s disease
* Auditory processing
* Cognition
* Dichotic Digits Test
|full-text-url=https://sci-hub.do/10.1159/000506621
}}
{{medline-entry
|title=Uptake kinetics of four hydrophobic organic pollutants in the earthworm Eisenia andrei in aged laboratory-contaminated natural soils.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/32061977
|abstract=Laboratory studies of pollutant uptake kinetics commonly start shortly after experimental soil contamination when it is not clear if the processes between soil and chemicals are equilibrated and stabilized. For instance, when the concentration in soil quickly decreases due to initial biodegradation, bioaccumulation may show a peak-shape accumulation curve instead of conventional first order kinetics with a plateau at the end. The results of such experiments with soil freshly contaminated in the laboratory are then hardly comparable to bioaccumulation observed in soils from historically contaminated sites. Therefore, our study focused on the uptake kinetics of four hydrophobic organic compounds (pyrene, lindane, p,p'-[[DDT]] and PCB 153) in two laboratory-contaminated natural soils with different soil properties (e.g. total organic carbon content of 1.6 and 9.3%) aged for 203 days to mimic long-term contamination. For pyrene, the results surprisingly showed peak-shape accumulation curves despite long aging. It seems compound biodegradation might be significant in aged soils when the conditions change (e.g. by distribution to the experimental vessels) and this should be also considered when testing historically contaminated soils. For lindane, longer aging seems to guarantee stability of the soil-compound-earthworm system and the steady state was reached after 5 days of exposure. Furthermore, although concentrations of p,p'-[[DDT]] and PCB 153 in earthworms after 11-15-day exposure did not statistically differ, which is a commonly-used indicator that a steady state was reached, they continuously increased until the end of the exposure. Therefore, despite the aging, longer exposure was probably needed to reach the true equilibrium between concentrations in earthworms and soil. In summary, aging does not warranty the conventional first order kinetic curve with the equilibrium at the end of the exposure but may have diverse effects for compounds with different environmental properties and should be taken into account in the bioaccumulation factor calculation and the risk assessment.
|mesh-terms=* Animals
* DDT
* Hexachlorocyclohexane
* Hydrophobic and Hydrophilic Interactions
* Kinetics
* Oligochaeta
* Polychlorinated Biphenyls
* Pyrenes
* Soil Pollutants
|keywords=* Aging
* BAFs
* Bioaccumulation
* HOCs
* Laboratory-contaminated soils
|full-text-url=https://sci-hub.do/10.1016/j.ecoenv.2020.110317
}}
{{medline-entry
|title=Adult exposure to insecticides causes persistent behavioral and neurochemical alterations in zebrafish.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/31911208
|abstract=Farmers are often chronically exposed to insecticides, which may present health risks including increased risk of neurobehavioral impairment during adulthood and across aging. Experimental animal studies complement epidemiological studies to help determine the cause-and-effect relationship between chronic adult insecticide exposure and behavioral dysfunction. With the zebrafish model, we examined short and long-term neurobehavioral effects of exposure to either an organochlorine insecticide, dichlorodiphenyltrichloroethane ([[DDT]]) or an organophosphate insecticide chlorpyrifos (CPF). Adult fish were exposed continuously for either two or 5 weeks (10-30 nM [[DDT]], 0.3-3 μM CPF), with short- and long-term effects assessed at 1-week post-exposure and at 14 months of age respectively. The behavioral test battery included tests of locomotor activity, tap startle, social behavior, anxiety, predator avoidance and learning. Long-term effects on neurochemical indices of cholinergic function were also assessed. Two weeks of [[DDT]] exposure had only slight effects on locomotor activity, while a longer five-week exposure led to hypoactivity and increased anxiety-like diving responses and predator avoidance at 1-week post-exposure. When tested at 14 months of age, these fish showed hypoactivity and increased startle responses. Cholinergic function was not found to be significantly altered by [[DDT]]. The two-week CPF exposure led to reductions in anxiety-like diving and increases in shoaling responses at the 1-week time point, but these effects did not persist through 14 months of age. Nevertheless, there were persistent decrements in cholinergic presynaptic activity. A five-week CPF exposure led to long-term effects including locomotor hyperactivity and impaired predator avoidance at 14 months of age, although no effects were apparent at the 1-week time point. These studies documented neurobehavioral effects of adult exposure to chronic doses of either organochlorine or organophosphate pesticides that can be characterized in zebrafish. Zebrafish provide a low-cost model that has a variety of advantages for mechanistic studies and may be used to expand our understanding of neurobehavioral toxicity in adulthood, including the potential for such toxicity to influence behavior and development during aging.

|keywords=* Aging
* Anxiety-related behavior
* DDT
* Neurobehavioral toxicology
* Zebrafish
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061078
}}
{{medline-entry
|title=Second generation effects of larval metal pollutant exposure on reproduction, longevity and insecticide tolerance in the major malaria vector Anopheles arabiensis (Diptera: Culicidae).
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/31910892
|abstract=Members of the Anopheles gambiae complex breed in clean, sunlit temporary bodies of water. Anthropogenic pollution is, however, altering the breeding sites of the vectors with numerous biological effects. Although the effects of larval metal pollution have previously been examined, this study aims to assess the transgenerational effects of larval metal pollution on the major malaria vector An. arabiensis. Two laboratory strains of An. arabiensis, SENN (insecticide-susceptible) and SENN-[[DDT]] (insecticide-resistant), were used in this study. After being bred in water polluted with either cadmium chloride, copper nitrate or lead nitrate, several life history characteristics that can have epidemiological implications (fertility, apoptotic damage to reproductive structures, adult longevity and insecticide tolerance) were examined in the adults and compared to those of adults bred in clean water. All metal treatments reduced fecundity in SENN, but only lead treatment reduced fertility in SENN-[[DDT]]. Cadmium chloride exposure resulted in apoptosis and deformation of the testes in both strains. After breeding generation F0 in polluted water, F1 larvae bred in clean water showed an increase in longevity in SENN-[[DDT]] adult females. In contrast, after breeding the F0 generation in polluted water, longevity was reduced after cadmium and copper exposure in the F1 generation. Larval metal exposure resulted in an increase in insecticide tolerance in adults of the SENN strain, with SENN-[[DDT]] adults gaining the greatest fold increase in insecticide tolerance. This study demonstrates that a single exposure to metal pollution can have transgenerational effects that are not negated by subsequent breeding in clean water.
|mesh-terms=* Animals
* Anopheles
* Drug Resistance
* Female
* Fertility
* Insecticides
* Larva
* Male
* Metals
* Reproduction
* Water Pollutants
|keywords=* Anopheles arabiensis
* Insecticide resistance
* Longevity
* Transgenerational effects
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947826
}}
{{medline-entry
|title=Protective effect of Pedro-Ximénez must against p,p'-DDE-induced liver damages in aged Mus spretus mice.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/31765701
|abstract=Aging is characterized by deterioration of biomolecules and impaired stress responses that make the elderly especially vulnerable to environmental pollutants. The pesticide p,p'-DDE is a [[DDT]] derivative that generates great concern because of its wide distribution and its harmful effects on both human health and the environment. We analyzed here the biological responses elicited by p,p'-DDE exposure in the liver of aged Mus spretus mice. Data demonstrate that the elderly constitute a population especially sensitive to this noxious environmental pollutant. We also demonstrated here that the daily consumption of sun-dried Pedro Ximénez (PX) white-grape must (PXM) protects the liver of aged mice from both the age and the damages caused by p,p'-DDE exposure. The PXM activity was exerted through the restoration of the hepatic metabolisms of lipids and carbohydrates and, probably, is a consequence of the ability of this polyphenol-rich mixture to avoid oxidative stress. Nutritional interventions including PXM, which ameliorates the effects of unavoidable exposure to pesticides in our food, are helpful tools that can help elderly populations to enjoy a healthy and expanded lifetime.
|mesh-terms=* Aging
* Animals
* Antioxidants
* Chemical and Drug Induced Liver Injury
* Dichlorodiphenyl Dichloroethylene
* Down-Regulation
* Liver
* Male
* Mice
* Oxidative Stress
* Pesticides
* Plant Extracts
* Polyphenols
* Transcriptome
* Up-Regulation
* Vitis
|keywords=* Aging
* Hepatoprotection
* Mus spretus
* Organochlorine
* Oxidative damage
* Pedro-ximénez grape must
* Transcriptional analysis
* p,p'-DDE
|full-text-url=https://sci-hub.do/10.1016/j.fct.2019.110984
}}
{{medline-entry
|title=Effects of inorganic fertilizer on larval development, adult longevity and insecticide susceptibility in the malaria vector Anopheles arabiensis (Diptera: Culicidae).
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/31713993
|abstract=Exposure to inorganic fertilizer is common for the major malaria vector Anopheles arabiensis, which is closely associated with agricultural activities. The aim of this study was to understand if insecticide susceptible and resistant individuals respond to fertilizer exposure in the same manner. Two laboratory strains, SENN, an insecticide susceptible strain, and SENN-[[DDT]], an insecticide resistant strain selected strain selected from SENN, were used in this study. Both strains were exposed to one of three concentrations of a combination nitrogen-phosphorus-potassium (NPK) inorganic fertilizer, as well as nitrogenous (urea), phosphorus (superphosphate) and kaelic (potassium chloride, KCl) elemental fertilizer. The time to pupation was monitored, adult longevity was assessed and the insecticide tolerance of adults was determined. The effect of elemental fertilizers on ovipositioning site choice was also assessed. For both strains, urea increased the number of eggs laid, while superphosphate resulted in a significant decrease in egg laying. Larval NPK exposure decreased the time to pupation in the SENN strain but not in SENN-[[DDT]]. Urea exposure increased the time to pupation in both strains, while KCl decreased the time to pupation in both strains. Larval NPK exposure only affected adult male longevity at high concentrations. Larval exposure to NPK and KCl resulted in increased insecticide tolerance in both strains, with variable efficacy from strain to strain. Exposure to inorganic fertilizers has a greater effect on insecticide susceptible An. arabiensis as compared to resistant strains, where the primary advantage is increased insecticide tolerance. These data also demonstrate that larval fertilizer exposure can affect fecundity and fertility, and alter the life histories of adult An. arabiensis. © 2019 Society of Chemical Industry.
|mesh-terms=* Animals
* Anopheles
* Fertilizers
* Insecticide Resistance
* Insecticides
* Longevity
* Malaria
* Male
* Mosquito Vectors
|keywords=* Anopheles
* inorganic fertilizer
* insecticide resistance
* life history
* malaria
|full-text-url=https://sci-hub.do/10.1002/ps.5676
}}
{{medline-entry
|title=Low-dose endosulfan inhibits proliferation and induces senescence and pro-inflammatory cytokine production in human lymphocytes, preferentially impacting cytotoxic cells.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/31589084
|abstract=Endosulfan is a [[DDT]]-era organochlorine pesticide. Due to past and current environmental contamination, investigation of endosulfan exposure is of current importance. Acute high dose exposure precipitates neural/endocrine system damage, but the effects on the immune system and of lower doses are not well-characterized. Two relatively low concentrations of endosulfan (i.e. 0.1 and 17 µM ENDO) were investigated in an [i]in vitro[/i] study using human peripheral blood mononuclear cells (PBMC) to understand effects of relatively low doses (0.1-25.0 µM [≈0.04-10 ppm/40-10,000 ppb]) of ENDO upon normal human T- and B-lymphocytes and NK cells. The study here found that 17 µM ENDO inhibited phytohemagglutinin-M (PHA)-induced human PBMC proliferation. It was also seen that senescence and apoptosis among non-stimulated cells was increased, specifically within CD8 and NK populations, and that CD4:CD8 ratios also were increased. Treatment of non-stimulated PBMC with ENDO led to overall increases in production of tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-2, -4, and -6, and decreased production of anti-inflammatory IL-10, suggesting an immunosenescence secretory phenotype. Interestingly, when the cells were pre-stimulated with mitogen (PHA), ENDO became inhibitory against the mitogen-induced proliferation and cytokine formation - with the exception of that of TNFα and IL-6, suggesting differential effects of ENDO on activated cells. Thus, at the organismal level, ENDO might also display differential effects during states of autoimmune disease or chronic viral infection in the exposed host.
|mesh-terms=* Adult
* B-Lymphocytes
* Cell Proliferation
* Cells, Cultured
* Cellular Senescence
* Cytokines
* Dose-Response Relationship, Drug
* Endosulfan
* Female
* Healthy Volunteers
* Humans
* Inflammation Mediators
* Insecticides
* Killer Cells, Natural
* Male
* Primary Cell Culture
* T-Lymphocytes, Cytotoxic
* Young Adult
|keywords=* Endosulfan
* Immunosenescence
* NK cells
* PBMC
* cytotoxic cells
* interferon
* organochlorine pesticide
* senescence
|full-text-url=https://sci-hub.do/10.1080/1547691X.2019.1668513
}}
{{medline-entry
|title=DNA damage tolerance in stem cells, ageing, mutagenesis, disease and cancer therapy.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/31251805
|abstract=The DNA damage response network guards the stability of the genome from a plethora of exogenous and endogenous insults. An essential feature of the DNA damage response network is its capacity to tolerate DNA damage and structural impediments during DNA synthesis. This capacity, referred to as DNA damage tolerance ([[DDT]]), contributes to replication fork progression and stability in the presence of blocking structures or DNA lesions. Defective [[DDT]] can lead to a prolonged fork arrest and eventually cumulate in a fork collapse that involves the formation of DNA double strand breaks. Four principal modes of [[DDT]] have been distinguished: translesion synthesis, fork reversal, template switching and repriming. All [[DDT]] modes warrant continuation of replication through bypassing the fork stalling impediment or repriming downstream of the impediment in combination with filling of the single-stranded DNA gaps. In this way, [[DDT]] prevents secondary DNA damage and critically contributes to genome stability and cellular fitness. [[DDT]] plays a key role in mutagenesis, stem cell maintenance, ageing and the prevention of cancer. This review provides an overview of the role of [[DDT]] in these aspects.
|mesh-terms=* Adaptation, Physiological
* Aging
* Animals
* DNA Damage
* DNA Repair
* Humans
* Models, Genetic
* Mutagenesis
* Neoplasms
* Stem Cells

|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698745
}}
{{medline-entry
|title=The contribution of gut bacteria to insecticide resistance and the life histories of the major malaria vector Anopheles arabiensis (Diptera: Culicidae).
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/31235803
|abstract=The gut microbiota of mosquitoes is a crucial determinant of their fitness. As such, the biology of the gut microbiota of Anopheles arabiensis, a major malaria vector of Southern Africa, was investigated. Two laboratory strains of An. arabiensis were used; SENN, an insecticide susceptible strain, and SENN-[[DDT]], a resistant strain. The strains were supplemented with either non-commensal bacteria or antibiotics via a sucrose source to sterilize the gut. The strains were fed the broad-spectrum bactericidal antibiotic gentamicin, or a preferentially gram-positive bactericidal (vancomycin), gram-negative bactericidal (streptomycin) or broad-spectrum bacteriostatic (erythromycin), either by sugar supplementation or by artificially-spiked blood-meal. The effects on adult mosquito longevity and insecticide resistance phenotype were assessed. Bacteria from the midgut of both strains were characterised by MALDI-TOF mass spectroscopy. Bactericidal antibiotics increased longevity in SENN-[[DDT]]. Bacterial supplementation increased insecticide tolerance. Antibiotic supplementation via sugar decreased tolerance to the insecticides deltamethrin and malathion. Blood-supplemented vancomycin decreased insecticide resistance, while gentamicin and streptomycin increased resistance. SENN showed a greater gut bacterial diversity than SENN-[[DDT]], with both strains dominated by Gram-negative bacteria. This study suggests a crucial role for bacteria in An. arabiensis life history, and that gut microflora play variable roles in insecticide resistant and susceptible mosquitoes.
|mesh-terms=* Animals
* Anopheles
* Anti-Bacterial Agents
* Gastrointestinal Microbiome
* Insecticide Resistance
* Longevity
* Malaria
* Mosquito Vectors
* Phenotype

|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591418
}}
{{medline-entry
|title=Exposure to the insecticide-treated bednet PermaNet 2.0 reduces the longevity of the wild African malaria vector Anopheles funestus but GSTe2-resistant mosquitoes live longer.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/30870507
|abstract=Despite the increased report of insecticide resistance in malaria vectors, its impact on mosquito's life-traits after exposure to insecticide-treated nets remains under investigated. Here, we assessed the effects of exposure to PermaNet 2.0 on several life traits of An. gambiae s.l. and An. funestus s.l. field mosquitoes in Cameroon. Female Anopheles mosquitoes were collected indoor using electric aspirators in southern Cameroon (Obout) in 2016. After assessing the resistance status of F1 from the field collected-mosquitoes, progeny of the first generation (An. funestus s.l.) and seventh generation (An. gambiae s.l.) were used to assess the long-term effect of exposure to PermaNet 2.0 on several life-traits of these vectors (longevity, blood feeding ability, fecundity and fertility) in comparison to untreated net. In addition, the L119F-GSTe2 mutation associated with [[DDT]]/pyrethroids resistance in An. funestus was genotyped to assess its association with increased life-span post-exposure. Both An. funestus and An. gambiae were resistant to pyrethroids and [[DDT]] with a greater level in the latter. Pyrethroid-only nets PermaNet 2.0 (17.5% mortality) and Olyset (0% mortality) exhibited a significantly reduced efficacy against An. funestus in contrast to a greater efficacy for PBO-based Nets Olyset Plus (65% mortality), PermaNet 3.0 top (100% mortality). In both species, mosquitoes that survived exposure to PermaNet 2.0 exhibited a significantly reduced longevity than those non-exposed (6.95 days vs 12.46 for An. funestus P<0.001; 8.87 vs 11.25 days for An. gambiae; P<0.001). However, no significant difference was observed for blood feeding and fecundity in both species. In addition, molecular analysis of the L119F-GSTe2 mutation revealed that this mutation is associated with an increase in the chance of surviving after exposure to this net in An. funestus. These results show that although the PermaNet 2.0 presents a reduced efficacy against resistant populations, it remains efficient after exposure by reducing the life expectancy of the vectors which could contribute in the reduction of malaria incidence.
|mesh-terms=* Animals
* Anopheles
* Cameroon
* Female
* Glutathione Transferase
* Humans
* Insect Proteins
* Insecticide Resistance
* Insecticide-Treated Bednets
* Insecticides
* Longevity
* Malaria
* Mosquito Control
* Mosquito Vectors
* Mutation, Missense
* Pyrethrins

|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417719
}}
{{medline-entry
|title=The occurrence and dynamics of polychlorinated hydrocarbons in roe deer ([i]Capreolus capreolus[/i]) in South-western Slovakia.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/30729857
|abstract=The goal of this study was to determined polychlorinated biphenyls (PCBs) and organochlorine pesticides in the depot fat of roe deer ([i]Capreolus capreolus[/i]) coming from south-western Slovakia. The mutual correlations of the organic pollutants were analyzed. The study included dichlorodiphenyltrichloroethane ([[DDT]]), hexachlorobenzen (HCB), alpha-hexachlorocyclohexane and beta-hexachlorocyclohexane (α   β-HCH), gamma-hexachlorocyclohexane (γ-HCH), and polychlorinated biphenyls (PCB-delor). The gas chromatograph with an electron capture detector [[ECD]] was used for analysis. The accumulations of organic pollutant in depot fat of roe deer were in following order: [[DDT]] > PCB-delor > α   β-HCH > HCB > γ-HCH. Among all pollutants, [[DDT]] was accumulated significantly in the highest level in the samples. The significantly higher content of [[DDT]], HCB, α   β-HCH, and γ-HCH was detected in the adult animals when compared to the juveniles. Some strong positive correlations among pollutants, between HCB and [[DDT]], α   β-HCH and HCB, α   β-HCH and HCB, between γ-HCH and other pollutants, and between PCB-delor and γ-HCH were found. Game animals are a part of human food chain and monitoring of the environment pollution by PCBs and other organic pollutants are worthy to study.
|mesh-terms=* Adipose Tissue
* Aging
* Animals
* Deer
* Environmental Monitoring
* Environmental Pollutants
* Female
* Humans
* Hydrocarbons, Chlorinated
* Male
* Pesticides
* Polychlorinated Biphenyls
* Sex Factors
* Slovakia
|keywords=* Slovakia
* depot fat
* organic pollution
* polychlorinated hydrocarbon
* roe deer
|full-text-url=https://sci-hub.do/10.1080/10934529.2019.1574152
}}
{{medline-entry
|title=Insecticide resistance status of three vectors of Japanese encephalitis in east central India.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/30565276
|abstract=Japanese encephalitis (JE) has been reported in different districts of Odisha state (east central India) since 1992. During 2016, a major outbreak of JE and acute encephalitis syndrome (AES) occurred in the Malkangiri district of Odisha, causing 103 deaths in children, of which 37 were caused by JE and 66 by AES. Information on insecticide resistance in JE vectors is important for the selection of appropriate insecticides for use in vector control. The present study was designed to determine the resistance status of three important vectors of JE, Culex vishnui, Culex tritaeniorhynchus and Culex bitaeniorhynchus (Diptera: Culicidae), against dichlorodiphenyltrichloroethane ([[DDT]]), malathion and deltamethrin in three districts of Odisha state affected by JE. Female adult mosquitoes were collected using mouth aspirators both indoors and outdoors in JE-affected villages and used in susceptibility bioassays following World Health Organization guidelines. Knock-downs were recorded every 10 min up to 1 h and mortality rates were recorded at 24 h post-exposure. Culex vishnui and Cx. tritaeniorhynchus showed resistance to [[DDT]], malathion and deltamethrin, whereas Cx. bitaeniorhynchus was susceptible in all study districts. The information generated by this study will be highly useful in the planning and implementing of appropriate vector control operations for the prevention and control of JE in east central India.
|mesh-terms=* Animals
* Culex
* DDT
* Encephalitis Virus, Japanese
* Encephalitis, Japanese
* Female
* India
* Insecticide Resistance
* Insecticides
* Longevity
* Malathion
* Mosquito Control
* Mosquito Vectors
* Nitriles
* Pyrethrins
|keywords=* Insecticide resistance
* JE vectors
* Odisha
* WHO susceptibility bioassays
* knock-down time
|full-text-url=https://sci-hub.do/10.1111/mve.12352
}}
{{medline-entry
|title=Effect of aging on bioaccessibility of [[DDT]]s and PCBs in marine sediment.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/30471469
|abstract=Hydrophobic legacy contaminants like dichlorodiphenyltrichloroethane ([[DDT]]) and polychlorinated biphenyls (PCBs) were banned almost half a century ago. While their residues still remain in many environmental compartments, they have undergone extensive aging and likely have lower bioaccessibility (the available fraction) compared to fresh residues. However, risk assessment relies heavily on the use of total chemical concentration, rather than accounting for age-diminished bioaccessibility, likely leading to overestimated risks. In this study, we used 24 h Tenax desorption to measure the potential bioaccessibility of [[DDT]]s and PCBs in two sediment cores taken from the Palos Verdes Shelf Superfund site in the Pacific Ocean. The total concentrations of [[DDT]]s and PCBs from the core located at the sewage outfall (8C) were as high as 41,000-15,700 μg/kg (dry weight, dw) and 530-2600 μg/kg dw, respectively, while those from a location 7 km northeast of the outfall (3C) were 2-3 orders of magnitude lower. Bioaccessibility estimated by 24-h Tenax-aided desorption (F ) decreased in the order of DDD > DDE > [[DDT]] for [[DDT]] derivatives, and PCB 52 > PCB 70 > PCB 153 for PCB congeners, showing a negative correlation with their log K . Due to the extensive aging, F  values were <20% of the total chemical concentration for most contaminants and <5% for [[DDT]], DDE and PCB 153, suggesting that aging greatly diminished their bioavailability. However, a quantitative relationship between F  and sediment age along the vertical profile was not found, likely because the contaminant residues had undergone aging before their offsite transport and deposition onto the ocean floor. As the use of man-made chemicals such as [[DDT]] and PCBs was discontinued in the U.S. many decades ago, the reduction in their bioavailability due to aging may be universal and should be taken into consideration to avoid overly conservative risk predictions or unnecessary mitigation interventions.
|mesh-terms=* Animals
* DDT
* Geologic Sediments
* Pacific Ocean
* Polychlorinated Biphenyls
* Sewage
* Time Factors
* Water Pollutants, Chemical
|keywords=* Aging effect
* Bioavailability
* Hydrophobic organic contaminants
* Legacy contaminants
* Tenax desorption
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6349416
}}
{{medline-entry
|title=The effect of commercial herbicide exposure on the life history and insecticide resistance phenotypes of the major malaria vector Anopheles arabiensis (Diptera: culicidae).
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/30179608
|abstract=Herbicides, such as atrazine and glyphosate, are common agrochemicals known to pollute surface ground water. As such, aquatic invertebrates associated with agricultural activities can be exposed to varying doses of these xenobiotics. Anopheles arabiensis, a major malaria vector species in southern Africa, is often closely associated with agricultural activities. This study aimed to examine the effects of larval atrazine or glyphosate exposure on larval and adult life history traits on two laboratory strains of An. arabiensis; one insecticide susceptible (SENN), the other selected for resistance (SENN [[DDT]]). Atrazine delayed time to pupation in both strains, but markedly more so in SENN [[DDT]]. Glyphosate treatment reduced time to pupation in SENN [[DDT]]. Larval atrazine exposure decreased adult longevity in SENN, while both herbicide treatments significantly increased adult longevity in SENN [[DDT]]. Larval glyphosate exposure was the more potent enhancer of insecticide tolerance in adult mosquitoes. In SENN [[DDT]], it reduced deltamethrin and malathion-induced mortality, and the LT50 s for these insecticides were increased in association with herbicide exposure. Glyphosate exposure also increased the LT50 s for malathion and deltamethrin in SENN. Exposure to both herbicides had contrasting effects on detoxification enzyme activities. Although both increased cytochrome P450 activity, they had opposite effects on those enzymes involved in reactive oxygen species detoxification. Glyphosate decreased glutathione S-transferase activity, but increased catalase activity with atrazine having the opposite effect. This study demonstrates that larval exposure to the herbicides atrazine and glyphosate can affect the insecticide susceptibilities and life history traits of epidemiological importance in An. arabiensis, with glyphosate being the more potent effector of insecticide resistance.
|mesh-terms=* Animals
* Anopheles
* Disease Vectors
* Herbicides
* Insecticide Resistance
* Larva
* Longevity
* Malaria
* Mosquito Vectors
* Nitriles
* Phenotype
* Pyrethrins
|keywords=* Anopheles arabiensis
* Herbicides
* Insecticide resistance
|full-text-url=https://sci-hub.do/10.1016/j.actatropica.2018.08.030
}}
{{medline-entry
|title=Is Chronic Exposure to Low-Dose Organochlorine Pesticides a New Risk Factor of T-cell Immunosenescence?
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/29991517
|abstract= T-cell immunosenescence, a hallmark of an aging immune system, is potentially linked to the risk of developing cancer and other aging-related diseases. Chronic infection by cytomegalovirus (CMV) has been widely studied as a risk factor for T-cell immunosenescence, but the role of persistent chemicals has never been examined. As a typical example of persistent chemicals, we evaluated whether organochlorine pesticides (OCPs) are related to T-cell immunosenescence in the general population.  Serum concentrations of β-hexachlorocyclohexane, [i]p,p[/i]'-[[DDT]], [i]p,p[/i]'-DDE, and trans-nonachlor were measured in 95 Korean adults ages 30 to 64 years. T-cell immunosenescence was assessed by the frequencies of CD8 CD57 , CD8 [[CD28]] , [[CD4]] CD57 , and [[CD4]] [[CD28]]  T lymphocytes in 20 mL of fresh peripheral blood.  The senescence of CD8  T lymphocytes was the most consistently associated with OCPs. For quartiles of measurements of OCPs, adjusted mean percentages of CD8 CD57  and CD8 [[CD28]]  T lymphocytes in the CD8  T lymphocyte population were 23.9, 27.6, 31.0, and 38.7 ([i]P[/i]   < 0.01) and 25.6, 27.3, 28.0, and 35.5 ([i]P[/i]   = 0.02), respectively. When we compared the strength of the associations among OCPs, CMV IgG titer, and age, OCPs showed the strongest association with markers of immunosenescence. Importantly, the association between OCPs and immunosenescence markers was more prominent among participants without known risk factors, such as a young age or low CMV immunoglobulin G titer.  Chronic exposure to low-dose OCPs may be a new risk factor for T-cell immunosenescence.  T-cell immunosenescence may be one possible mechanism linking low-dose OCPs and many chronic diseases. [i]Cancer Epidemiol Biomarkers Prev; 27(10); 1159-67. ©2018 AACR[/i].
|mesh-terms=* Adult
* Female
* Follow-Up Studies
* Hexachlorocyclohexane
* Humans
* Immunosenescence
* Male
* Middle Aged
* Pesticides
* Prognosis
* Risk Factors
* T-Lymphocytes

|full-text-url=https://sci-hub.do/10.1158/1055-9965.EPI-17-0799
}}
{{medline-entry
|title=The influence of age on insecticide susceptibility of Anopheles arabiensis during dry and rainy seasons in rice irrigation schemes of Northern Tanzania.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/28893240
|abstract=Insecticide resistance is the major emerging challenge facing the malaria vector control programmes in Tanzania. Proper monitoring and detection is of paramount importance guiding the vector control programmes. This paper presents the effect of mosquito aging on insecticide resistance status in Anopheles arabiensis populations in dry and rainy seasons in northern Tanzania. Anopheles gambiae s.l. larvae were sampled from rice fields in both dry and rainy seasons and reared in the insectary to adults. The emerged females in batches of 2, 3, 5, and 10 days old were exposed to six insecticides (deltamethrin, permethrin, lambda-cyhalothrin, [[DDT]], bendiocarb and pirimiphos-methyl) to see the effects of age on insecticide resistance. Mosquitoes were exposed to insecticides using WHO standard susceptibility test kits. Knockdown was recorded during the 1-h exposure, while mortality and resistance ratio were recorded 24 h later. Mosquito specimens were identified to species level using the polymerase chain reaction (PCR) method. Among the 326 specimens processed by PCR, 323 (99.1%) were identified as Anopheles arabiensis. There was reduced mortality (ranging from 61 to 97.7%) when adults reared from larvae were exposed to all pyrethroids and bendiocarb in both dry and rainy seasons, while they were fully susceptible to [[DDT]] and pirimiphos-methyl. There was a significant increase in mortality rate with increase in mosquito's age in both dry and rainy seasons following exposure to pyrethroids (DF = 1, P < 0.05). Mosquitoes showed significantly higher mortality rates in the rainy season than in the dry season after being exposed to pyrethroids (DF = 1, P < 0.05). Higher mortality rates (94.0-99.8%) were observed in all ages and seasons when mosquitoes were exposed to bendiocarb compared with pyrethroids. Pirimiphos-methyl was only tested in the rainy season so no comparison with dry season mosquitoes could be made. Results showed that An. arabiensis were resistant to pyrethroids in both seasons and that the young age groups exhibited higher levels of resistance compared with the older age groups. Mosquitoes were full susceptible to [[DDT]] and pirimiphos-methyl irrespective of the season and age.
|mesh-terms=* Aging
* Agricultural Irrigation
* Animals
* Anopheles
* Insecticide Resistance
* Insecticides
* Oryza
* Seasons
* Tanzania
|keywords=* Age
* Anopheles arabiensis
* Insecticides
* Pyrethr
* Resistance
* Seasons
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5594483
}}
{{medline-entry
|title=DNA damage tolerance in hematopoietic stem and progenitor cells in mice.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/28761001
|abstract=DNA damage tolerance ([[DDT]]) enables bypassing of DNA lesions during replication, thereby preventing fork stalling, replication stress, and secondary DNA damage related to fork stalling. Three modes of [[DDT]] have been documented: translesion synthesis (TLS), template switching (TS), and repriming. TLS and TS depend on site-specific [[PCNA]] K164 monoubiquitination and polyubiquitination, respectively. To investigate the role of [[DDT]] in maintaining hematopoietic stem cells (HSCs) and progenitors, we used [i]Pcna[/i]  mice as a unique [[DDT]]-defective mouse model. Analysis of the composition of HSCs and HSC-derived multipotent progenitors (MPPs) revealed a significantly reduced number of HSCs, likely owing to increased differentiation of HSCs toward myeloid/erythroid-associated MPP2s. This skewing came at the expense of the number of lymphoid-primed [[MPP4]]s, which appeared to be compensated for by increased [[MPP4]] proliferation. Furthermore, defective [[DDT]] decreased the numbers of MPP-derived common lymphoid progenitor (CLP), common myeloid progenitor (CMP), megakaryocyte-erythroid progenitor (MEP), and granulocyte-macrophage progenitor (GMP) cells, accompanied by increased cell cycle arrest in CMPs. The HSC and MPP phenotypes are reminiscent of premature aging and stressed hematopoiesis, and indeed progressed with age and were exacerbated on cisplatin exposure. Bone marrow transplantations revealed a strong cell intrinsic defect of [[DDT]]-deficient HSCs in reconstituting lethally irradiated mice and a strong competitive disadvantage when cotransplanted with wild-type HSCs. These findings indicate a critical role of [[DDT]] in maintaining HSCs and progenitor cells, and in preventing premature aging.
|mesh-terms=* Aging
* Animals
* Cell Differentiation
* DNA Damage
* DNA Repair
* DNA Replication
* Hematopoiesis
* Hematopoietic Stem Cells
* Mice, Inbred C57BL
* Mice, Knockout
* Multipotent Stem Cells
* Proliferating Cell Nuclear Antigen
|keywords=* DNA damage tolerance
* hematopoietic stem and progenitor cells
* proliferating cell nuclear antigen K164 ubiquitination
* template switching
* translesion synthesis
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5565453
}}
{{medline-entry
|title=The effects of ingestion of hormonal host factors on the longevity and insecticide resistance phenotype of the major malaria vector Anopheles arabiensis (Diptera: Culicidae).
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/28700639
|abstract=Exogenous vertebrate-derived factors circulating in the blood have the capacity to modulate the biology of haematophagous insects. These include insulin, insulin growth factor 1 (IGF) and transforming growth factor β1 (TGFβ). The effects of the consumption of these three proteins were examined on laboratory strains of Anopheles arabiensis. SENN, an insecticide susceptible strain and SENN [[DDT]], a resistant strain selected from SENN, were fed with host factor-supplemented sucrose. Adult longevity was measured and insecticide resistance phenotype over time was assessed by WHO bioassay. Detoxification and oxidative stress defence enzyme activity was assessed calorimetrically. Insulin supplementation augmented insecticide resistance in young adult mosquitoes. This effect was due to the hormonal nature of the protein, as heat-denatured insulin did not elicit the same response. In contrast, IGF and TGFβ consumption generally reduced the expression of insecticide resistance. Insulin ingestion significantly reduced longevity in the insecticide susceptible strain. IGF elicited the same response in the susceptible strain, while TGF consumption had no effect on either strain. Consumption of all factors significantly decreased Glutathione S-transferase activity and increased cytochrome P450 and superoxide dismutase activity. This suggests that the altered detoxification phenotype is mediated primarily by cytochrome P450 activity, which would result in an increase in oxidative stress. The increased superoxide dismutase activity suggests that this enzyme class alleviates the oxidative stress as opposed to glutathione-based redox systems. Oxidative stress responses play a crucial role in insecticide resistance and longevity. These data show that ingested hormonal factors can affect mosquito longevity and insecticide susceptibility, both of which are important characteristics in terms of malaria transmission and control.
|mesh-terms=* Animals
* Anopheles
* Biological Assay
* Cytochrome P-450 Enzyme System
* Female
* Glutathione Transferase
* Insect Vectors
* Insecticide Resistance
* Longevity
* Malaria
* Oxidative Stress
* Transforming Growth Factor beta1

|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507448
}}
{{medline-entry
|title=Bioavailability of five hydrophobic organic compounds to earthworms from sterile and non-sterile artificial soils.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/28371706
|abstract=Bioaccumulation factors (BAFs) of organic pollutants to soil biota, often required by risk assessment, are mostly obtained in non-sterile laboratory-contaminated artificial soils. However, microbial degradation has been indicated by many authors to influence the fate of hydrophobic organic compounds (HOCs) in soils. A question arises if the microbial community of peat which is used for artificial soil preparation affects the measured values of BAFs. In this study the effect of soil microorganisms on bioavailability of HOCs was studied and a portion of each soil was sterilized by gamma irradiation. Results indicated that the sterilization process significantly affected the fate of polycyclic aromatic hydrocarbons (PAHs; phenanthrene and pyrene) and increased bioavailability of these compounds to earthworms with BAFs several times higher in the sterile soils compared to their non-sterile variants. This suggests that sterilization of soils can be used as the "worst-case scenario" for laboratory tests of toxicity or bioaccumulation of biodegradable HOCs such as PAHs. It represents a situation of limited microbial degradation resulting in higher bioavailable fractions to other organisms (e.g. invertebrates). This may be the case in soils where microbial communities face stresses caused by contamination or land management. The bioavailability of chlorinated HOCs (lindane, 4,4'-[[DDT]] and PCB 153) was not affected by sterilization, as their BAFs were similar in the sterile and non-sterile soils during the experiment.
|mesh-terms=* Animals
* Biological Availability
* Hydrophobic and Hydrophilic Interactions
* Oligochaeta
* Organic Chemicals
* Polycyclic Aromatic Hydrocarbons
* Soil
* Soil Microbiology
* Soil Pollutants
* Sterilization
|keywords=* Aging
* Artificial soil
* Bioaccumulation factors
* Organic carbon content
* Sterilization
|full-text-url=https://sci-hub.do/10.1016/j.chemosphere.2017.03.117
}}
{{medline-entry
|title=The effect of elevated temperatures on the life history and insecticide resistance phenotype of the major malaria vector Anopheles arabiensis (Diptera: Culicidae).
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/28193292
|abstract=Temperature plays a crucial role in the life history of insects. Recent climate change research has highlighted the importance of elevated temperature on malaria vector distribution. This study aims to examine the role of elevated temperatures on epidemiologically important life-history traits in the major malaria vector, Anopheles arabiensis. Specifically, the differential effects of temperature on insecticide-resistant and susceptible strains were examined. Two laboratory strains of A. arabiensis, the insecticide-susceptible SENN and the insecticide-resistant SENN [[DDT]] strains, were used to examine the effect of elevated temperatures on larval development and adult longevity. The effects of various elevated temperatures on insecticide resistance phenotypes were also examined and the biochemical basis of the changes in insecticide resistance phenotype was assessed. SENN and SENN [[DDT]] larvae developed at similar rates at elevated temperatures. SENN [[DDT]] adult survivorship did not vary between control and elevated temperatures, while the longevity of SENN adults at constantly elevated temperatures was significantly reduced. SENN [[DDT]] adults lived significantly longer than SENN at constantly elevated temperatures. Elevated rearing temperatures, as well as a short-term exposure to 37 and 39 °C as adults, augmented pyrethroid resistance in adult SENN [[DDT]], and increased pyrethroid tolerance in SENN. Detoxification enzyme activity was not implicated in this phenotypic effect. Quercertin-induced synergism of inducible heat shock proteins negated this temperature-mediated augmentation of pyrethroid resistance. Insecticide-resistant A. arabiensis live longer than their susceptible counterparts at elevated temperatures. Exposure to heat shock augments pyrethroid resistance in both resistant and susceptible strains. This response is potentially mediated by inducible heat shock proteins.
|mesh-terms=* Animals
* Anopheles
* Female
* Hot Temperature
* Insect Vectors
* Insecticide Resistance
* Insecticides
* Larva
* Life History Traits
* Male
* Phenotype
|keywords=* Adult longevity
* Cross-tolerance
* Heat shock protein
* Insecticide resistance
* Larval development rate
* Thermal stress
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307775
}}
{{medline-entry
|title=Dichotic Digits Test Performance Across the Ages: Results From Two Large Epidemiologic Cohort Studies.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/27941404
|abstract=The Dichotic Digits test ([[DDT]]) has been widely used to assess central auditory processing but there is limited information on observed [[DDT]] performance in a general population. The purpose of the study was to determine factors related to [[DDT]] performance in a large cohort spanning the adult age range. The study was cross-sectional and subjects were participants in the Epidemiology of Hearing Loss Study (EHLS), a population-based investigation of age-related hearing loss, or the Beaver Dam Offspring Study (BOSS), a study of aging in the adult offspring of the EHLS members. Subjects seen during the 4th EHLS (2008 to 2010) or the 2nd BOSS (2010 to 2013) examination were included (N = 3655 participants [1391 EHLS, 2264 BOSS]; mean age = 61.1 years, range = 21 to 100 years). The free and right ear-directed recall [[DDT]]s were administered using 25 sets of triple-digit pairs with a 70 dB HL presentation level. Pure-tone audiometric testing was conducted and the pure-tone threshold average (PTA) at 0.5, 1, 2, and 4 kHz was categorized using the worse ear: no loss = PTA ≤ 25 dB HL; mild loss = 25 < PTA ≤ 40 dB HL; moderate or marked loss = PTA > 40 dB HL. Cognitive impairment was defined as a Mini-Mental State Examination score < 24 (maximum = 30) or a self- or proxy-reported history of dementia or Alzheimer's disease. Demographic information was self-reported. General linear models were fit and multiple linear regression was performed. The mean total free recall [[DDT]] score was 76.7% (range = 21.3 to 100%). Less than 10% of the participants had a total free recall score below 60% correct. The mean right ear-directed recall score was 98.4% with 69% of the participants scoring 100% and another 15.5% scoring 98.7% (1 incorrect digit). In multivariable modeling of the total free recall scores, the predicted mean free recall score was 1 percentage point lower for every 5-year increase in age, 2.3 percentage points lower in males than females, 8.7 percentage points lower in participants with less than a high school degree than in those with college degrees, 6.8 percentage points lower in participants with a moderate or marked hearing loss compared with no hearing loss, and 8.3 percentage points lower in participants with cognitive impairment compared with those without cognitive impairment. These 5 factors were independently and significantly related to performance and accounted for 22.7% of the total variability in free recall scores. Substantial variation in the total free recall [[DDT]] scores but very little variation in the right ear-directed recall [[DDT]] scores was observed. Age, sex, education, hearing loss severity, and cognitive impairment were found to be significantly related to [[DDT]] scores but explained less than 25% of the total variability in total free recall scores. The right ear-directed recall [[DDT]] by itself may not be of benefit in assessing central auditory processing in a general population because of its limited variability but further evaluation of factors potentially related to free recall [[DDT]] variability may prove useful.
|mesh-terms=* Adult
* Aged
* Aged, 80 and over
* Aging
* Audiometry, Pure-Tone
* Auditory Perception
* Cohort Studies
* Cross-Sectional Studies
* Dichotic Listening Tests
* Epidemiologic Studies
* Female
* Hearing Disorders
* Humans
* Linear Models
* Male
* Middle Aged
* Young Adult

|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404957
}}
{{medline-entry
|title=The Role of Oxidative Stress in the Longevity and Insecticide Resistance Phenotype of the Major Malaria Vectors Anopheles arabiensis and Anopheles funestus.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/26964046
|abstract=Oxidative stress plays numerous biological roles, both functional and pathological. The role of oxidative stress in various epidemiologically relevant biological traits in Anopheles mosquitoes is not well established. In this study, the effects of oxidative stress on the longevity and insecticide resistance phenotype in the major malaria vector species An. arabiensis and An. funestus were examined. Responses to dietary copper sulphate and hydrogen peroxide were used as proxies for the oxidative stress phenotype by determining the effect of copper on longevity and hydrogen peroxide lethal dose. Glutathione peroxidase and catalase activities were determined colorimetrically. Oxidative burden was quantified as protein carbonyl content. Changes in insecticide resistance phenotype were monitored by WHO bioassay. Insecticide resistant individuals showed an increased capacity for coping with oxidative stress, mediated by increased glutathione peroxidase and catalase activity. This effect was observed in both species, as well as in laboratory strains and F1 individuals derived from wild-caught An. funestus mothers. Phenotypic capacity for coping with oxidative stress was greatest in strains with elevated Cytochrome P450 activity. Synergism of oxidative stress defence enzymes by dietary supplementation with haematin, 3-Amino-1, 2, 4-triazole and Sodium diethyldithiocarbamate significantly increased pyrethroid-induced mortality in An. arabiensis and An. funestus. It is therefore concluded that defence against oxidative stress underlies the augmentation of the insecticide resistance phenotype associated with multiple blood-feeding. This is because multiple blood-feeding ultimately leads to a reduction of oxidative stress in insecticide resistant females, and also reduces the oxidative burden induced by [[DDT]] and pyrethroids, by inducing increased glutathione peroxidase activity. This study highlights the importance of oxidative stress in the longevity and insecticide resistance phenotype in malaria vectors. 
|mesh-terms=* Animals
* Anopheles
* Copper Sulfate
* Feeding Behavior
* Female
* Hydrogen Peroxide
* Insect Vectors
* Insecticide Resistance
* Insecticides
* Kaplan-Meier Estimate
* Longevity
* Male
* Oxidative Stress
* Phenotype
* Plasmodium

|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786153
}}
{{medline-entry
|title=Nuclear hormone receptor DHR96 mediates the resistance to xenobiotics but not the increased lifespan of insulin-mutant Drosophila.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/26787908
|abstract=Lifespan of laboratory animals can be increased by genetic, pharmacological, and dietary interventions. Increased expression of genes involved in xenobiotic metabolism, together with resistance to xenobiotics, are frequent correlates of lifespan extension in the nematode worm Caenorhabditis elegans, the fruit fly Drosophila, and mice. The Green Theory of Aging suggests that this association is causal, with the ability of cells to rid themselves of lipophilic toxins limiting normal lifespan. To test this idea, we experimentally increased resistance of Drosophila to the xenobiotic dichlordiphenyltrichlorethan ([[DDT]]), by artificial selection or by transgenic expression of a gene encoding a cytochrome P450. Although both interventions increased [[DDT]] resistance, neither increased lifespan. Furthermore, dietary restriction increased lifespan without increasing xenobiotic resistance, confirming that the two traits can be uncoupled. Reduced activity of the insulin/Igf signaling (IIS) pathway increases resistance to xenobiotics and extends lifespan in Drosophila, and can also increase longevity in C. elegans, mice, and possibly humans. We identified a nuclear hormone receptor, DHR96, as an essential mediator of the increased xenobiotic resistance of IIS mutant flies. However, the IIS mutants remained long-lived in the absence of DHR96 and the xenobiotic resistance that it conferred. Thus, in Drosophila IIS mutants, increased xenobiotic resistance and enhanced longevity are not causally connected. The frequent co-occurrence of the two traits may instead have evolved because, in nature, lowered IIS can signal the presence of pathogens. It will be important to determine whether enhanced xenobiotic metabolism is also a correlated, rather than a causal, trait in long-lived mice. 
|mesh-terms=* Animals
* Drosophila
* Drosophila Proteins
* Drug Resistance
* Insulin
* Life Expectancy
* Mutation
* Receptors, Cytoplasmic and Nuclear
* Transcription, Genetic
* Xenobiotics
|keywords=* DHR96
* IIS
* lifespan
* nuclear hormone receptor
* xenobiotic resistance
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747718
}}
{{medline-entry
|title=Influence of feeding and earthworm density on compound bioaccumulation in earthworms Eisenia andrei.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/26378968
|abstract=Earthworm density and feeding during exposure to contaminated soil have been used inconsistently in bioaccumulation studies, which may lead to possible errors in risk assessment and modeling. Hydrophobic organic pollutants with a wide range of environmental properties (phenanthrene, pyrene, lindane, p,p'-[[DDT]], and PCB 153) were used to study the effect of different earthworm densities in combination with the presence or absence of feeding on bioaccumulation factors (BAFs). Similar BAFs were found at various soil-to-worm ratios, with the exception of phenanthrene. We recommend using at least 15 gsoil dw per earthworm. The absence of feeding doubled the BAFs and, thus, using no food ration can be considered as "the worst case scenario". Whenever food is to be applied (i.e. to ensure the validity of the test in earthworm mass loss), we suggest feeding depending on the organic carbon content of the studied soil.
|mesh-terms=* Animals
* DDT
* Feeding Behavior
* Hexachlorocyclohexane
* Oligochaeta
* Phenanthrenes
* Polychlorinated Biphenyls
* Population Density
* Pyrenes
* Soil
* Soil Pollutants
|keywords=* Aging
* Artificial soil
* Bioaccumulation factors
* Bioavailability
* Hydrophobic organic compounds
|full-text-url=https://sci-hub.do/10.1016/j.envpol.2015.09.025
}}
{{medline-entry
|title=Greater cognitive decline with aging among elders with high serum concentrations of organochlorine pesticides.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/26107947
|abstract=Although cognitive decline is very common in elders, age-related cognitive decline substantially differs among elders and the determinants of the differences in age-related cognitive decline are unclear. We investigated our hypothesis that the association between age and cognition was stronger in those with higher serum concentrations of organochlorine (OC) pesticides, common persistent and strongly lipophilic neurotoxic chemicals. Participants were 644 elders aged 60-85, participating in the National Health and Nutrition Examination Survey 1999-2002. Six OC pesticides (p,p'-dichlorodiphenyltrichloroethane ([[DDT]]), p,p'-dichlorodipenyldichloroethylene (DDE), β-hexachlorocyclohexane, trans-nonachlor, oxychlordane, and heptachlor epoxide) were evaluated. "Lower cognitive function" was defined as having a low Digit-Symbol Substitution Test (DSST) score (<25th percentile of DSST score, cutpoint 28 symbols substituted). Higher levels of β-hexachlorocyclohexane, trans-nonachlor, oxychlordane, and heptachlor epoxide modified the associations between age and lower cognitive function (Pinteraction<0.01, 0.03, <0.01, and 0.02, respectively). Elders in the 3rd tertile of these chemicals demonstrated a greater risk of lower cognitive function with aging, compared to those in the combined 1st and 2nd tertiles. Among those with highest OC pesticides (3rd tertile), the odds ratio for the risk of lower cognitive function was about 6 to 11 for the highest quintile of age (80-85 years) vs. the first quintile of age (60-63 years), while the association between age and lower cognitive function became flatter in those with lower OC pesticides (combined 1st and 2nd tertiles). Both [[DDT]] and DDE showed no interaction, with lower DSST scores for higher age irrespective of serum concentrations of [[DDT]] or DDE. Even though DSST score measures only one aspect of cognition, several OC pesticides modified aging-related prevalence of low cognitive score, a finding which should be evaluated in prospective studies. 
|mesh-terms=* Aged
* Aged, 80 and over
* Aging
* Chlordan
* Cognition
* Cognition Disorders
* DDT
* Dichlorodiphenyl Dichloroethylene
* Environmental Exposure
* Female
* Heptachlor Epoxide
* Hexachlorocyclohexane
* Humans
* Hydrocarbons, Chlorinated
* Male
* Middle Aged
* Nutrition Surveys
* Odds Ratio
* Pesticides
* Risk

|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4480979
}}
{{medline-entry
|title=The effect of multiple blood-feeding on the longevity and insecticide resistant phenotype in the major malaria vector Anopheles arabiensis (Diptera: Culicidae).
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/25150975
|abstract=Anopheles arabiensis is a major malaria vector in Africa. Adult females are likely to imbibe multiple blood meals during their lifetime. This results in regular exposure to potential toxins and blood-meal induced oxidative stress. Defence responses to these stressors may affect other factors of epidemiological significance, such as insecticide resistance and longevity. The aims of this study were to examine the effect of multiple blood-feeding on insecticide tolerance/resistance with increasing age, to assess the underlying biochemical mechanisms for the responses recorded, and to assess the effect of multiple blood-feeding on the life histories of adult females drawn from insecticide resistant and susceptible laboratory reared An. arabiensis. Laboratory reared An. arabiensis females from an insecticide resistant and an insecticide susceptible colony were offered either a single blood meal or multiple blood meals at 3-day intervals. Their tolerance or resistance to insecticide was then monitored by WHO bioassay four hours post blood-feeding. The biochemical basis of the phenotypic response was assessed by examining the effect of blood on detoxification enzyme activity and the effect of blood-meals on detoxification enzyme activity in ageing mosquitoes. Control cohorts that were not offered any blood meals showed steadily decreasing levels of insecticide tolerance/resistance with age, whereas a single blood meal significantly increased tolerance/resistance primarily at the age of three days. The expression of resistance/tolerance in those cohorts fed multiple blood meals generally showed the least variation with age. These results were consistent following exposure to [[DDT]] and pyrethroids but not to malathion. Multiple blood-meals also maintained the [[DDT]] and permethrin resistant phenotype, even after treatment females had stopped taking blood-meals. Biochemical analysis suggests that this phenotypic effect in resistant females may be mediated by the maintenance of increased glutathione s-transferase activity as a consequence of multiple blood-feeding. Multiple blood-feeding increased the longevity of insecticide resistant females regardless of their mating status, but only increased the longevity of unmated susceptible females. These data suggest that multiple blood-feeding confers a competitive advantage to insecticide resistant females by increased longevity and maintenance of the expression of resistance with age.
|mesh-terms=* Aging
* Animals
* Anopheles
* Blood
* Feeding Behavior
* Female
* Insecticide Resistance
* Insecticides
* Longevity

|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161849
}}
{{medline-entry
|title=The role of canine distemper virus and persistent organic pollutants in mortality patterns of Caspian seals (Pusa caspica).
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/24987857
|abstract=Persistent organic pollutants are a concern for species occupying high trophic levels since they can cause immunosuppression and impair reproduction. Mass mortalities due to canine distemper virus (CDV) occurred in Caspian seals (Pusa caspica), in spring of 1997, 2000 and 2001, but the potential role of organochlorine exposure in these epizootics remains undetermined. Here we integrate Caspian seal mortality data spanning 1971-2008, with data on age, body condition, pathology and blubber organochlorine concentration for carcases stranded between 1997 and 2002. We test the hypothesis that summed PCB and [[DDT]] concentrations contributed to CDV associated mortality during epizootics. We show that age is the primary factor explaining variation in blubber organochlorine concentrations, and that organochlorine burden, age, sex, and body condition do not account for CDV infection status (positive/negative) of animals dying in epizootics. Most animals (57%, n = 67) had PCB concentrations below proposed thresholds for toxic effects in marine mammals (17 µg/g lipid weight), and only 3 of 67 animals had predicted TEQ values exceeding levels seen to be associated with immune suppression in harbour seals (200 pg/g lipid weight). Mean organonchlorine levels were higher in CDV-negative animals indicating that organochlorines did not contribute significantly to CDV mortality in epizootics. Mortality monitoring in Azerbaijan 1971-2008 revealed bi-annual stranding peaks in late spring, following the annual moult and during autumn migrations northwards. Mortality peaks comparable to epizootic years were also recorded in the 1970s-1980s, consistent with previous undocumented CDV outbreaks. Gompertz growth curves show that Caspian seals achieve an asymptotic standard body length of 126-129 cm (n = 111). Males may continue to grow slowly throughout life. Mortality during epizootics may exceed the potential biological removal level (PBR) for the population, but the low frequency of epizootics suggest they are of secondary importance compared to anthropogenic sources of mortality such as fishing by-catch.
|mesh-terms=* Age Factors
* Aging
* Animals
* Biometry
* Body Burden
* Body Constitution
* Distemper
* Distemper Virus, Canine
* Environmental Exposure
* Female
* Humans
* Hydrocarbons, Chlorinated
* Male
* RNA, Viral
* Reverse Transcriptase Polymerase Chain Reaction
* Seals, Earless
* Seasons
* Sex Factors
* Water Pollutants, Chemical

|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079250
}}
{{medline-entry
|title=Solid phase microextraction of organic pollutants from natural and artificial soils and comparison with bioaccumulation in earthworms.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/24433790
|abstract=The presented study investigates the use of passive sampling, i.e. solid phase microextraction with polydimethylsiloxane fibers (PDMS-SPME), to assess the bioavailability of fiver neutral organic chemicals (phenanthrene, pyrene, lindane, p,p'-[[DDT]], and PCB 153) spiked to natural and artificial soils after different aging times. Contaminant bioavailability was assessed by comparing PDMS concentrations with results from a 10 day bioaccumulation test with earthworms (Eisenia fetida). The hypotheses tested were (i) organic carbon (OC) normalization, which is commonly used to account for sorption and bioavailability of hydrophobic organic chemicals in soil risk assessment, has limitations due to differences in sorptive properties of OC and aging processes (i.e. sequestration and biodegradation) and (ii) PDMS-SPME provides a more reliable measure of soil contaminant bioavailability than OC normalized soil concentrations. The above stated hypotheses were confirmed since the results showed that: (i) the PDMS/soil organic carbon partition ratio (R) accounting for the role that OC plays in partitioning significantly differed between soils and aging times and (ii) the correlation with earthworm concentrations was better using porewater concentrations derived from PDMS concentrations than when organic normalized soil concentrations were used. Capsule: Sorption of organic compounds measured by SPME method and their bioavailability to earthworms cannot be reliably predicted using OC content. 
|mesh-terms=* Animals
* Environmental Monitoring
* Oligochaeta
* Organic Chemicals
* Soil Pollutants
* Solid Phase Microextraction
|keywords=* Aging
* Artificial soil
* Bioaccumulation
* Solid phase micro-extraction (SPME)
* Total organic carbon content (TOC)
|full-text-url=https://sci-hub.do/10.1016/j.ecoenv.2013.11.011
}}
{{medline-entry
|title=Concentrations and potential health hazards of organochlorine pesticides in (shallow) groundwater of Taihu Lake region, China.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/24239826
|abstract=A total of 27 shallow groundwater samples were collected from the Taihu Lake region (TLR), to determine the concentrations of 14 organochlorine pesticide (OCP) species, identify their possible sources, and estimate health risk of drinking the shallow groundwater. All OCP species occurred in the shallow groundwater of TLR with high detection frequency except p, p'-dichlorodiphenyldichlorothane (p, p'-DDD) and p, p'-dichlorodiphenyltrichloroethane (p, p'-[[DDT]]). [[DDT]]s and hexachlorocyclohexanes (HCHs) were the dominant OCP contaminants in the shallow groundwater of TLR, and they account for 44.2% total OCPs. The low α-HCH/γ-HCH ratio, high β-HCH/(α γ)-HCH ratio and β-HCH being the dominant HCH isomers for the majority of samples suggest that the HCHs were mainly from the historical use of lindane after a period of degradation. p, p'-DDE being the dominant [[DDT]] metabolite for all the samples indicated that the [[DDT]]s were mainly from the historical residues. Compositional analysis also suggested that there were fresh input sources of heptachlors, aldrins and endrins in addition to the historical residues. Correlation analysis indicated the hexachlorobenzene (HCB) impurity in the shallow groundwater of TLR was likely from the historical application of lindane and technical HCH (a mixture of HCH isomers that is produced by photochlorination of benzene). Carcinogenic risk values for α-HCH, heptachlor, heptachlor epoxide, aldrins and dieldrin in the shallow groundwater in majority area of TLR were found to be >10(-6), posing a potentially serious cancer risk to those dependant on shallow groundwater for drinking water. 
|mesh-terms=* China
* Groundwater
* Hazardous Substances
* Humans
* Hydrocarbons, Chlorinated
* Lakes
* Pesticides
* Water Pollutants, Chemical
|keywords=* AT
* Aldrins
* BW
* C
* CDI
* CV
* Cancer risk
* Composition
* DDT
* DDTs
* ED
* EF
* Endrins
* GC-μECD
* HCB
* HCH
* HCHs
* HPLC
* HQ
* Heptachlors
* IR
* MDL
* OCP
* Organochlorine pesticides
* POPs
* PTFE
* PVC
* R
* R(f)D
* S/N
* SF
* SPE
* Shallow groundwater
* Source identification
* TLR
* Taihu Lake region
* US EPA
* United State Environmental Protection Agency
* aldrin and dieldrin
* average lifespan
* body weight
* carcinogenic risk
* chemical concentration in water
* chronic daily intake
* coefficient of variation
* dichlorodiphenyltrichloroethane
* endrin and endrin aldehyde
* exposure duration
* gas chromatography equipped with a (63)Ni electron capture detector
* hazard quotient
* heptachlor and heptachlor epoxide
* hexachlorobenzene
* hexachlorocyclohexane
* high performance liquid chromatography
* method detection limit
* organochlorine pesticide
* p, p′-DDD
* p, p′-DDE
* p, p′-DDE, p, p′-DDD and p, p′-DDT
* p, p′-dichlorodiphenyldichlorothane
* p, p′-dichlorodiphenylethane
* persistent organic pollutants
* polytetrafluoroethylene
* polyvinyl chloride
* reference dose of the contaminant via oral exposure
* signal versus noise value
* slope factor of the contaminant via oral exposure route
* solid phase extraction
* the exposure frequency
* water ingestion rate
* α-, β-, γ-, δ-isomers
|full-text-url=https://sci-hub.do/10.1016/j.scitotenv.2013.10.056
}}