Редактирование: COQ3

Ubiquinone biosynthesis O-methyltransferase, mitochondrial precursor (3-demethylubiquinol 3-O-methyltransferase) (EC 2.1.1.64) (Polyprenyldihydroxybenzoate methyltransferase) (EC 2.1.1.114) [UG0215E05]

==Publications==

{{medline-entry
|title=Isolation and functional expression of human [[COQ3]], a gene encoding a methyltransferase required for ubiquinone biosynthesis.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/10777520
|abstract=The [[COQ3]] gene in Saccharomyces cerevisiae encodes an O-methyltransferase required for two steps in the biosynthetic pathway of ubiquinone (coenzyme Q, or Q). This enzyme methylates an early Q intermediate, 3,4-dihydroxy-5-polyprenylbenzoic acid, as well as the final intermediate in the pathway, converting demethyl-Q to Q. This enzyme is also capable of methylating the distinct prokaryotic early intermediate 2-hydroxy-6-polyprenyl phenol. A full-length cDNA encoding the human homologue of [[COQ3]] was isolated from a human heart cDNA library by sequence homology to rat Coq3. The clone contained a 933-base pair open reading frame that encoded a polypeptide with a great deal of sequence identity to a variety of eukaryotic and prokaryotic Coq3 homologues. In the region between amino acids 89 and 255 in the human sequence, the rat and human homologues are 87% identical, whereas human and yeast are 35% identical. When expressed in multicopy, the human construct rescued the growth of a yeast coq3 null mutant on a nonfermentable carbon source and restored coenzyme Q biosynthesis, although at lower levels than that of wild type yeast. In vitro methyltransferase assays using farnesylated analogues of intermediates in the coenzyme Q biosynthetic pathway as substrates showed that the human enzyme is active with all three substrates tested.
|mesh-terms=* Aging
* Amino Acid Sequence
* Animals
* Base Sequence
* Catechol O-Methyltransferase
* Chromatography, High Pressure Liquid
* DNA, Complementary
* Gene Library
* Humans
* Methyltransferases
* Models, Chemical
* Molecular Sequence Data
* Mutation
* Myocardium
* Rats
* Saccharomyces cerevisiae
* Sequence Homology, Amino Acid
* Time Factors
* Ubiquinone

|full-text-url=https://sci-hub.do/10.1074/jbc.275.17.12381
}}