Редактирование: CLK1

Dual specificity protein kinase CLK1 (EC 2.7.12.1) (CDC-like kinase 1) [CLK]

==Publications==

{{medline-entry
|title=Conservation of the Caenorhabditis elegans timing gene clk-1 from yeast to human: a gene required for ubiquinone biosynthesis with potential implications for aging.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/10501970
|abstract=Mutations in the Caenorhabditis elegans gene clk-1 have a major effect on slowing development and increasing life span. The Saccharomyces cerevisiae homolog [[COQ7]] encodes a mitochondrial protein involved in ubiquinone biosynthesis and, hence, is required for respiration and gluconeogenesis. In this study, RT-PCR and 5' RACE were used to isolate both human and mouse clk-1/[[COQ7]] homologs. Human CLK-1 was mapped to Chr 16(p12-13.1) by Radiation Hybrid (RH) and fluorescence in situ hybridization (FISH) methods. The number and location of human [[CLK1]] introns were determined, and the location of introns II and IV are the same as in C. elegans. Northern blot analysis showed that three different isoforms of CLK-1 mRNA are present in several tissues and that the isoforms differ in the amount of expression. The functional equivalence of human CLK-1 to the yeast [[COQ7]] homolog was tested by introducing either a single or multicopy plasmid containing human CLK-1 cDNA into yeast coq7 deletion strains and assaying for growth on a nonfermentable carbon source. The human CLK-1 gene was able to functionally complement yeast coq7 deletion mutants. The protein similarities and the conservation of function of the CLK-1/clk-1/[[COQ7]] gene products suggest a potential link between the production of ubiquinone and aging.
|mesh-terms=* Aging
* Alternative Splicing
* Amino Acid Sequence
* Animals
* Biological Clocks
* Caenorhabditis elegans
* Caenorhabditis elegans Proteins
* Chromosome Mapping
* Chromosomes, Human, Pair 16
* Cloning, Molecular
* Conserved Sequence
* Evolution, Molecular
* Exons
* Genetic Complementation Test
* Helminth Proteins
* Humans
* In Situ Hybridization, Fluorescence
* Introns
* Mice
* Molecular Sequence Data
* RNA, Messenger
* Sequence Homology, Amino Acid
* Ubiquinone
* Yeasts

|full-text-url=https://sci-hub.do/10.1007/s003359901147
}}