Редактирование: CHMP1B

Charged multivesicular body protein 1b (CHMP1.5) (Chromatin-modifying protein 1b) (CHMP1b) (Vacuolar protein sorting-associated protein 46-2) (Vps46-2) (hVps46-2) [C18orf2]

==Publications==

{{medline-entry
|title=Overexpression of [[CHMP7]] from rapeseed and Arabidopsis causes dwarfism and premature senescence in Arabidopsis.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/27497741
|abstract=Endosomal sorting complexes required for transport (ESCRT) are well known in mammalians and yeast and plays an essential role in the formation of multi-vesicular bodies. Accumulating evidence has shown that ESCRT proteins contribute to proper plant development. [[CHMP7]] (charged multi-vesicular body protein 7) is an ESCRT-III-related protein and functions in the endosomal sorting pathway in humans. However, its function in plants has not been explored in detail. In this study, we isolate the putative homolog of [[CHMP7]] from rapeseed, Bn[[CHMP7]], which contains eight exons and encodes a protein consisting of 423 amino acid residues. Compared with the wild-type, overexpression of Bn[[CHMP7]] in Arabidopsis disturbs plant growth and decreases seed yield. Moreover, the transgenic plants also display early leaf senescence and hypersensitivity to dark treatment due to defects in autophagic degradation. Further study showed that Bn[[CHMP7]] is highly expressed in leaves and that YFP-Bn[[CHMP7]] is predominantly localized in endosome. Compared with human [[CHMP7]], we found that Bn[[CHMP7]] not only interacts with ESCRT-III subunits SNF7.2 (CHMP4B), but also with VPS2.2 and [[CHMP1B]]. As expected, microarray analysis revealed that the expression of ESCRT transport genes is significantly affected. Additionally, the expression of some genes that are involved in senescence, protein synthesis and protein degradation is also altered in Bn[[CHMP7]]-overexpressing plants. Taken together, Bn[[CHMP7]] encodes an endosome-localized protein, which causes dwarfism and leaf senescence as an ESCRT-III-related component.
|mesh-terms=* Arabidopsis
* Autophagy
* Base Sequence
* Brassica rapa
* Endosomal Sorting Complexes Required for Transport
* Endosomes
* Gene Expression Profiling
* Gene Expression Regulation, Plant
* Genes, Plant
* Phenotype
* Plant Leaves
* Plant Proteins
* Plants, Genetically Modified
* Protein Binding
* Sequence Analysis, DNA
* Subcellular Fractions
|keywords=* CHMP7
* Dwarfism
* ESCRT-III
* Leaf senescence
* Rapeseed
|full-text-url=https://sci-hub.do/10.1016/j.jplph.2016.06.023
}}