Редактирование: ACAA2

3-ketoacyl-CoA thiolase, mitochondrial (EC 2.3.1.16) (Acetyl-CoA acetyltransferase) (EC 2.3.1.9) (Acetyl-CoA acyltransferase) (Acyl-CoA hydrolase, mitochondrial) (EC 3.1.2.-) (EC 3.1.2.1) (EC 3.1.2.2) (Beta-ketothiolase) (Mitochondrial 3-oxoacyl-CoA thiolase) (T1)

==Publications==

{{medline-entry
|title=p46Shc Inhibits Thiolase and Lipid Oxidation in Mitochondria.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/27059956
|abstract=Although the p46Shc isoform has been known to be mitochondrially localized for 11 years, its function in mitochondria has been a mystery. We confirmed p46Shc to be mitochondrially localized and showed that the major mitochondrial partner of p46Shc is the lipid oxidation enzyme 3-ketoacylCoA thiolase [[ACAA2]], to which p46Shc binds directly and with a strong affinity. Increasing p46Shc expression inhibits, and decreasing p46Shc stimulates enzymatic activity of thiolase in vitro Thus, we suggest p46Shc to be a negative mitochondrial thiolase activity regulator, and reduction of p46Shc expression activates thiolase. This is the first demonstration of a protein that directly binds and controls thiolase activity. Thiolase was thought previously only to be regulated by metabolite balance and steady-state flux control. Thiolase is the last enzyme of the mitochondrial fatty acid beta-oxidation spiral, and thus is important for energy metabolism. Mice with reduction of p46Shc are lean, resist obesity, have higher lipid oxidation capacity, and increased thiolase activity. The thiolase-p46Shc connection shown here in vitro and in organello may be an important underlying mechanism explaining the metabolic phenotype of Shc-depleted mice in vivo.
|mesh-terms=* Acetyl-CoA C-Acyltransferase
* Animals
* Binding, Competitive
* Blotting, Western
* Cell Line
* Energy Metabolism
* Fatty Acids
* HEK293 Cells
* HeLa Cells
* Humans
* Lipid Metabolism
* Mice
* Mice, Inbred C57BL
* Mice, Knockout
* Mitochondria
* Mitochondrial Proteins
* NIH 3T3 Cells
* Oxidation-Reduction
* Protein Binding
* Protein Isoforms
* RNA Interference
* Shc Signaling Adaptor Proteins
* Src Homology 2 Domain-Containing, Transforming Protein 1
|keywords=* Shc
* aging
* diet
* high fat diet resistance
* insulin
* lipid metabolism
* longevity
* mitochondria
* p46Shc
* thiolase
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933453
}}