Редактирование: AKIP1

A-kinase-interacting protein 1 (Breast cancer-associated gene 3 protein) (PKA-interacting protein) (Proline-rich protein BCA3) [BCA3] [C11orf17]

==Publications==

{{medline-entry
|title=Expression of potato RNA-binding proteins StUBA2a/b and StUBA2c induces hypersensitive-like cell death and early leaf senescence in Arabidopsis.
|pubmed-url=https://pubmed.ncbi.nlm.nih.gov/25944928
|abstract=The Arabidopsis thaliana genome encodes three RNA-binding proteins (RBPs), [[UBP1]]-associated protein 2a (UBA2a), UBA2b, and UBA2c, that contain two RNA-recognition motif (RRM) domains. They play important roles in wounding response and leaf senescence, and are homologs of Vicia faba abscisic-acid-activated protein kinase-interacting protein 1 (Vf[[AKIP1]]). The potato (Solanum tuberosum) genome encodes at least seven [[AKIP1]]-like RBPs. Here, two potato RBPs have been characterized, StUBA2a/b and StUBA2c, that are homologous to Vf[[AKIP1]] and Arabidopsis UBA2s. Transient expression of StUBA2s induced a hypersensitive-like cell death phenotype in tobacco leaves, and an RRM-domain deletion assay of StUBA2s revealed that the first RRM domain is crucial for the phenotype. Unlike overexpression of Arabidopsis UBA2s, constitutive expression of StUBA2a/b in Arabidopsis did not cause growth arrest and lethality at the young seedling stage, but induced early leaf senescence. This phenotype was associated with increased expression of defence- and senescence-associated genes, including pathogen-related genes (PR) and a senescence-associated gene (SAG13), and it was aggravated upon flowering and ultimately resulted in a shortened life cycle. Leaf senescence of StUBA2a/b Arabidopsis plants was enhanced under darkness and was accompanied by H2O2 accumulation and altered expression of autophagy-associated genes, which likely cause cellular damage and are proximate causes of the early leaf senescence. Expression of salicylic acid signalling and biosynthetic genes was also upregulated in StUBA2a/b plants. Consistent with the localization of UBA2s-GFPs and Vf[[AKIP1]]-GFP, soluble-modified GFP-StUBA2s localized in the nucleus within nuclear speckles. StUBA2s potentially can be considered for transgenic approaches to induce potato shoot senescence, which is desirable at harvest. 
|mesh-terms=* Amino Acid Sequence
* Arabidopsis
* Autophagy
* Cell Death
* Cloning, Molecular
* Gene Expression Regulation, Plant
* Genes, Plant
* Green Fluorescent Proteins
* Hydrogen Peroxide
* Molecular Sequence Data
* Phylogeny
* Plant Leaves
* Plant Proteins
* Plants, Genetically Modified
* RNA-Binding Proteins
* Solanum tuberosum
* Stress, Physiological
* Subcellular Fractions
* Tobacco
* Up-Regulation
|keywords=* AKIP proteins
* Arabidopsis thaliana
* RNA binding proteins
* Solanum tuberosum
* UBA2 proteins.
* senescence
|full-text-url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4473998
}}