Cryopreservation: различия между версиями

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812 байт убрано ,  19 июля 2022
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=== Perfusion ===
=== Perfusion ===


[[File:PerfusingPatient.jpg|thumb|Alcor contract surgeons prepare a patient for perfusion.]]


==== Vitrification ====
==== Vitrification ====
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===== M-22 =====
===== M-22 =====
[[File:M22 composition.jpg|400px|left]]


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=== Long Term Storage ===
=== Long Term Storage ===
[[File:CryoSpanSilos.jpg|300px|thumb|[[#CryoSpan|CryoSpan]] stored its patients in underground steel-reinforced concrete silos.]]


[[#Whole Body|Whole body]] [[#Patients|patients]] are stored head-down so that in the event of a leak, the head remains immersed, preventing thawing for some time while the problem is solved. More over, boil off of the Liquid Nitrogen happens continuously and the liquid is only replaced occasionally; because of this, parts of the patient could become exposed to Nitrogen vapor until it the dewar is replenished. This temperature changes can cause additional damage and fracturing from thermal stress.
[[#Whole Body|Whole body]] [[#Patients|patients]] are stored head-down so that in the event of a leak, the head remains immersed, preventing thawing for some time while the problem is solved. More over, boil off of the Liquid Nitrogen happens continuously and the liquid is only replaced occasionally; because of this, parts of the patient could become exposed to Nitrogen vapor until it the dewar is replenished. This temperature changes can cause additional damage and fracturing from thermal stress.
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==== Neurovault ====
==== Neurovault ====
[[File:Neurovault 1.jpg|thumb|right|Alcor's 'Cephalarium' (The neurovault) was a blast, earthquake and fire-proof concrete vault used to store a neuropatient dewar. The black rods on the first picture are fire-retardant fire logs.]]


The neurovault or 'cephalarium' used by Alcor was the world's first radiation, fire, earthquake and blast-resistant store of cryopatients.
The neurovault or 'cephalarium' used by Alcor was the world's first radiation, fire, earthquake and blast-resistant store of cryopatients.
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==== King Kong ====
==== King Kong ====
[[File:KingKong.jpg|thumb|left|TT's Jim Yount stands near the King Kong.]]


The King Kong Kapsule, built by TT engineer John Day, was the largest hard-vacuum dewar ever built, and was designed to hold eleven (Whole body) patients, even though its inside diameter was only 64 inches<ref>http://www.cs.cmu.edu/afs/cs/user/tsf/Public-Mail/cryonics/archive/333</ref>. The King Kong was put into service in 1990, when TT had enough patients to justify it. The dewar held eight patients, and never performed to specifications <ref>http://www.cryonet.org/cgi-bin/dsp.cgi?msg=5404</ref> <ref>http://www.cryonet.org/cgi-bin/dsp.cgi?msg=320</ref>. The Kong's performance was described as "subpar" <ref>John Day, TT's chief of engineering in the October, 1981 issue of Cryonics magazine. "Economics of surrounding a vacuum insulated dewar with additional foam insulation" CRYONICS pp. 13-18</ref> by Trans Time's engineer John Day, as its Liquid Nitrogen boiloff rate was 73 liters per day (1.5% of its capacity boiled off every day). When the Kapsule had 8 patients, this rate works out to a little over 9 liters of Liquid Nitrogen, every day, for every patient: A cost 300% greater than Alcor's<ref>http://www.cs.cmu.edu/afs/cs/user/tsf/Public-Mail/cryonics/archive/333</ref>. Because of allthese failures, they never payed more than half the contract price.
The King Kong Kapsule, built by TT engineer John Day, was the largest hard-vacuum dewar ever built, and was designed to hold eleven (Whole body) patients, even though its inside diameter was only 64 inches<ref>http://www.cs.cmu.edu/afs/cs/user/tsf/Public-Mail/cryonics/archive/333</ref>. The King Kong was put into service in 1990, when TT had enough patients to justify it. The dewar held eight patients, and never performed to specifications <ref>http://www.cryonet.org/cgi-bin/dsp.cgi?msg=5404</ref> <ref>http://www.cryonet.org/cgi-bin/dsp.cgi?msg=320</ref>. The Kong's performance was described as "subpar" <ref>John Day, TT's chief of engineering in the October, 1981 issue of Cryonics magazine. "Economics of surrounding a vacuum insulated dewar with additional foam insulation" CRYONICS pp. 13-18</ref> by Trans Time's engineer John Day, as its Liquid Nitrogen boiloff rate was 73 liters per day (1.5% of its capacity boiled off every day). When the Kapsule had 8 patients, this rate works out to a little over 9 liters of Liquid Nitrogen, every day, for every patient: A cost 300% greater than Alcor's<ref>http://www.cs.cmu.edu/afs/cs/user/tsf/Public-Mail/cryonics/archive/333</ref>. Because of allthese failures, they never payed more than half the contract price.
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==== Forever Flask ====
==== Forever Flask ====
[[File:Forever Flask deblasio.jpg|thumb|An overhead view of Ann DeBlasio being immersed in Liquid Nitrogen.]]


The 'Forever Flask' was the first dewar built by Minnesota Valley Engineering for the storage of cryopatients. It was made by welding together two standard A-8000 MVE dewars (Used for storage of biological specimens: Tissue cultures, bull semen of the Minnesota Valley Breeders Association), which had 25'' neck tube opening; wide enough, but not tall enough, for the storage of two human patients, but not tall enough.
The 'Forever Flask' was the first dewar built by Minnesota Valley Engineering for the storage of cryopatients. It was made by welding together two standard A-8000 MVE dewars (Used for storage of biological specimens: Tissue cultures, bull semen of the Minnesota Valley Breeders Association), which had 25'' neck tube opening; wide enough, but not tall enough, for the storage of two human patients, but not tall enough.
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== Emergency Procedures ==
== Emergency Procedures ==
[[File:CI LN tank.jpg|thumb|The Liquid Nitrogen storage tank used by the CI provides emergency replenishment capabilities.]]


At Alcor, in case of an emergency, all [[#Whole Body|whole body patients]] are to be converted to [[#Neuro|neuropatients]]. This would allow the current patient base of Alcor to be stored in two [[#Bigfoot|Bigfoot]] dewars.
At Alcor, in case of an emergency, all [[#Whole Body|whole body patients]] are to be converted to [[#Neuro|neuropatients]]. This would allow the current patient base of Alcor to be stored in two [[#Bigfoot|Bigfoot]] dewars.
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