CD6
T-cell differentiation antigen CD6 precursor (T12) (TP120) (CD6 antigen) [Contains: Soluble CD6]
Publications[править]
Aging is reflected by highly reproducible DNA methylation (DNAm) changes that open new perspectives for estimation of chronological age in legal medicine. DNA can be harvested non-invasively from cells at the inside of a person's cheek using buccal swabs - but these specimens resemble heterogeneous mixtures of buccal epithelial cells and leukocytes with different epigenetic makeup. In this study, we have trained an age predictor based on three age-associated CpG sites (associated with the genesPDE4C, ASPA, and ITGA2B) for swab samples to reach a mean absolute deviation (MAD) between predicted and chronological age of 4.3 years in a training set and of 7.03 years in a validation set. Subsequently, the composition of buccal epithelial cells versus leukocytes was estimated by two additional CpGs (associated with the genes CD6 and SERPINB5). Results of this "Buccal-Cell-Signature" correlated with cell counts in cytological stains (R2 = 0.94). Combination of cell type-specific and age-associated CpGs into one multivariate model enabled age predictions with MADs of 5.09 years and 5.12 years in two independent validation sets. Our results demonstrate that the cellular composition in buccal swab samples can be determined by DNAm at two cell type-specific CpGs to improve epigenetic age predictions.
MeSH Terms
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Aging
- Amidohydrolases
- Child
- Child, Preschool
- CpG Islands
- Cyclic Nucleotide Phosphodiesterases, Type 4
- DNA
- DNA Methylation
- Female
- Humans
- Infant
- Integrin alpha2
- Male
- Middle Aged
- Young Adult
Keywords
- aging
- cell composition
- epigenetic
- epithelial cells
- methylation
- predictor
- swab
Although there has been great progress in the characterization of alphabeta T cell differentiation, selection, and function, gammadelta T cells have remained poorly understood. One of the main reasons for this is the lack of gammadelta T cell-specific surface markers other than the TCR chains themselves. In this study we describe two novel surface receptors, SCART1 and SCART2. SCARTs are related to CD5, CD6, and CD163 scavenger receptors but, unlike them, are found primarily on developing and mature gammadelta T cells. Characterization of SCART2 positive immature and peripheral gammadelta T cells suggests that they undergo lineage specification in the thymus and belong to a new IL-17-producing subset with distinct homing capabilities.
MeSH Terms
- Aging
- Amino Acid Sequence
- Animals
- Cell Lineage
- Cell Movement
- Cells
- Cells, Cultured
- Dermis
- Down-Regulation
- Interleukin-17
- Lymph Nodes
- Mice
- Molecular Sequence Data
- Phylogeny
- Receptors, Antigen, T-Cell, gamma-delta
- Receptors, Cell Surface
- Sequence Alignment
- Signal Transduction
- Thymus Gland